(103) Olfactory Investigation of the Male is Stimulated by Administration of Dihydrotestosterone and Blunted by Co-administration of Flutamide in Ovariectomized Rats Primed with Estradiol

Abstract Introduction Although Hypoactive Sexual Desire Disorder (HSDD) is a common condition with relevant impact on women’s quality of life, approved androgenic formulations for its treatment are lacking. In previous studies, we demonstrated that the administration Dihydrotestosterone (DHT), non-aromatizable androgen, in ovariectomized (OVX) Long-Evans rats primed Estradiol Benzoate (EB), enhanced measures sexual behavior. Objective To further clarify mechanisms underlying facilitating action testosterone female function by investigating effect AR (androgen receptor) activation validated animal model. Methods After training and hormonal wash-out, OVX were treated different regimens: oil (O) + O (negative control group), 10 mcg EB+500 Progesterone (P) (positive DHT, DHT+10 mg flutamide (FLU), nonsteroidal antagonist (n=4 per group). EB was administered s.c. 48 h, P DHT 4 i.m. 24h prior to video-recorded behavioral testing sessions. These took place bisected unilevel pacing chambers, sexually experienced males. Five sessions conducted every 8 days. The main outcome appetitive behaviors: number hops/darts (partial solicitation), full solicitations olfactory investigations. Results A significant difference emerged among groups all measures. Regarding solicitations, animals EB+DHT displayed higher than negative group (O+O), although significantly lower positive (EB+P) (p=0.025). As novel finding, observed stimulating blunted EB+DHT+FLU, which showed behaviors (p=0.014 hops/darts, p=0.083, approaching statistical significance, solicitations). Interestingly, detected frequency investigations, displaying not only when compared O+O (p=0.047), but also (p=0.047 both comparisons). Once again, co-administration FLU reduced (p=0.005 investigations EB+DHT+FLU vs. EB+DHT). Conclusions data, be confirmed larger samples, indicate modulation exerted androgens behaviors, homologous interest women, independent aromatization. addition, specific Disclosure No